Table 1 of McBrien, Mol Vis 2009; 15:464-475.

Table 1. Muscarinic receptor subtype oligonucleotide primers and optimized real-time PCR conditions.



Receptor
subtype		 Oligonucleotide primers (5′-3′)

Size
(bp)		 Real-time PCR conditions


Forward

Reverse
MgCl2
(mM)		 Ann
temp
(°C)		 Det
temp
(°C)
CHRM1 GGAAGAGGAGAGGGATGAAGG TTCGGGAACACAGTCCTTG 365 2 63 89
CHRM2 TCATGACTGCGCTCTATTGG GGGCTTTTCCATTGTGGAT 197 3 60 82
CHRM3 GCCATCTTGTTCTGGCAATAC AGCGGCCATACTTCCTCC 325 2 60 82
CHRM4 ACACCGTGTACATCGTCAAG CAGGAGATGTGGATGTAGAGC 394 2 60 86
CHRM5 CCAACTCACAGGGCTTTTCT GTGAGCTGCTCGGCTTTG 173 3 60 87

Tree shrew-specific primers were designed for the five mAChR subtypes and were used to detect mRNA expression in the cornea, iris/ciliary body, retina, choroid, sclera, and scleral fibroblasts. The optimized conditions used to assess gene expression changes during myopia development are also included. Under “Real-time PCR conditions”, “Ann temp” stands for annealing temperature and “Det temp” stands for signal detection temperature.

McBrien, Mol Vis 2009; 15:464-475. http://www.molvis.org/molvis/v15/a47

©2009 Molecular Vision http://www.molvis.org/molvis/

ISSN 1090-0535