Figure 1. Muscarinic receptor subtype gene
expression in the tree shrew eye. RT–PCR and tree shrew-specific
primers were used to amplify mAChR subtypes in tree shrew
corneal, iris/ciliary body, retinal, choroidal, and scleral tissue
samples as well as in cultured scleral fibroblasts. Products
corresponding to the muscarinic receptor subtypes, CHRM1 (365
bp), CHRM2 (197 bp), CHRM3 (325 bp), CHRM4 (394
bp), and CHRM5 (173 bp), were amplified in most tissue cDNA
samples (+) while the corresponding total RNA was used to control for
genomic contamination (-). Nucleic acid size markers in base pairs are
indicated.