Figure 2. Immunohistochemical staining for COX-2, and survivin in control sections. Sections from Crohn’s disease (A) and human cutaneous malignant melanoma (C) were included as positive controls. No immunostaining for COX-2 (E) and survivin (F) in normal conjunctiva was observed. Sections incubated without a primary antibody (inset B) or with an isotype control antibody (inset D) displayed no immunoreactivity. Each section was counterstained with hematoxylin. Original magnification, A: 200X; C,E,F: 400X; insets B,D: 400X.