Figure 2. Alkaline phosphatase activity in
TR-iBRB2 cells. Effects of transfilter co-culture with TR-MUL5 cells (A)
and soluble factors secreted from TR-MUL5 cells (B) on the
activity of alkaline phosphatase in TR-iBRB2 cells. A: TR-MUL5
cells were situated on the basolateral aspect of TR-iBRB2 cell
monolayers as might occur in vivo and cells were co-cultured for six
days. B: TR-iBRB2 cells were cultured in the conditioned medium
of TR-MUL5 cells (MUL-CM) for 24 h. Each column represents the mean±SEM
(n=3). Asterisk represents p<0.01, significantly different from the
control.