Figure 9. Changes in GFAP expression after PEDF transfection. A,B: Reduced GFAP expression in DBA/2J mice retina and optic nerve, respectively, 4 months after AAV-PEDF transfection. C: Graphical representation of 3 independent experiments in A and B, normalized with actin loading control. As compared with AAV-GFP control, AAV-PEDF transduced mice show significantly reduced expression of GFAP in retina and optic nerve. ON: optic nerve. Statistical analysis for these experiments was performed using unpaired Student’s t-test (the asterisk indicates a p<0.05).