Figure 6. Localization of glial fibrillary acidic protein (GFAP) in retina and optic nerve from DBA/2J mice. A,B: Respective retina and optic nerve sections from DBA/2J mice at 1 month of age show minimal staining for GFAP. C,D: Respective retina and optic nerve sections from DBA/2J mice at 6 months of age show prominent immunostaining for GFAP in retinal Müller cells and optic nerve. E,F: western blot analysis for GFAP protein in the retina and optic nerve, respectively. G,H: Densitometric analysis of 3 independent experiments from E and F, using actin as a loading control. Statistical analyses for this experiment was performed using ANOVA with the Scheffé multiple comparison test (the asterisk indicates a p<0.05).