Figure 4 of Zhou, Mol Vis 2009; 15:438-450.


Figure 4. Increased TNF gene and protein expression in retina and optic nerve from DBA/2J mice. A: RT–PCR shows increased TNF gene expression in retina from DBA/2J mice at 3 and 6 months of age. B: Graphical representation of 3 independent experiments in A normalized with GAPDH loading control (mean±SD). C: Western blot shows increased TNF protein expression in retina from DBA/2J mice at 3 to 6 months of age. D: Densitometric analysis of 3 independent experiments from C, using actin as a loading control. E: Western blot shows increased TNF protein expression in optic nerve from DBA/2J mice from 3 to 6 months of age. F: Densitometric analysis of three independent experiments in E. Statistical analyses for this experiment was performed using ANOVA with the Scheffé multiple comparison test (the asterisk indicates a p<0.05).