Table 1 of Martin, Mol Vis 2009; 15:362-372. Table 1 of Martin, Mol Vis 2009; 15:362-372.
Table 1. Sequences of RT–PCR primers.
Gene name |
Primer sequence |
Expected product size (bp) |
|---|---|---|
| Human GPR109A (HM74A) | F: GGACAACTATGTGAGGCGTTGG | 650 |
| R: GGGCTGGAGAAGTAGTACACC | ||
| Human GPR109B (HM74) | F: CGTGATGGACTACTATGTGCG | 280 |
| R: ATTTGCAGGGCCATTCTGGAT | ||
| Mouse GPR109A (mPUMA-G) | F: GTTACAACTTCAGGTGGCACGAT | 443 |
| R: CTCCACACTAGTGCTTCGGTTATT | ||
| Rat GPR109A (rPUMA-G) | F: ACTTTCTGGTGATAAACGGCAAGA | 455 |
| R: GACTGTCAGGCCGATGGTG | ||
| HPRT1 | F: GCGTCGTGATTAGCGATGATGAAC | 150 |
| R: CCTCCCATCTCCTTCATGACATCT | ||
| 18S | Universal
Standard purchased from Applied Biosystems/Ambion (Austin, TX) Cat #: AM1716 |
315 |