Figure 6. Light micrographs and immunofluorescent staining. A,B,C,D: Retina of the non affected dog NA2. E,F,G,H: Retina of the 2 months old affected dog A6. B,F: Rod labeling with anti-Rho4D2 antibody. D,H: Cone labeling with PNA-FITC lectin. These results show that both the rods and cones are still present at the age of 2 months in affected dogs. Abbreviations: retinal pigment epithelium (RPE); outer segments (OS); inner segments (IS); outer nuclear layer (ONL); outer plexiform layer (OPL); inner nuclear layer (INL); inner plexiform layer (IPL); ganglion cell layer (GCL). The scale bar represents 50 µm.