Figure 3. Fold difference of S100A4, S100A6, S100A8, S100A9, and S100A11 gene transcripts in pterygium tissue relative to conjunctiva tissue. Total RNA was extracted from uninvolved conjunctiva and pterygium tissue after mechanical homogenization. Reverse transcription for 0.5 μg of total RNA. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control. ΔC_t was calculated by subtracting the C_t of GAPDH from the C_t of the targeted gene. The fold change was determined by the ΔΔC_t method (see text). The uninvolved conjunctiva sample was used as a reference for each of the tested S100 transcripts in the pterygium sample. Height of the bars represents mean value and error bars represent standard deviation. An asterisk indicates that p is less than 0.05 (p<0.05).