Figure 1. Cultured lenses exposed to hyperoxia in a sealed chamber. A: Lens culture chamber scheme: Lens (L) is totally immersed in culture medium (M). The chamber is placed vertically, with a covered top (T). B: Sealed chamber scheme: The cylinder supplied oxygen (99%) into the sealed chamber containing the culture chambers without a covered top, and the oxygen saturation inside the sealed chamber was kept above 80%.