Figure 5. NFκB p65 is indispensable for
IL-8 but dispensable for MCP-1 induction. A: A representative
western blot showed knockdown of NFκB p65. B: A luciferase
assay demonstrates reduction of IL-8 transcription to mock levels at
all times post infection by p65 siRNA treatment (p<0.0001). C:
MCP-1 ELISA revealed statistically increased expression at 4 h post
infection (p<0.0001), but transfected siRNA specific to p65 did not
alter MCP-1 expression, as compared to scrambled control siRNA.