Figure 3. Nuclear translocation of NFκB subunits upon viral infection. Confocal microscopy shows cellular localization of NFκB subunits. A: Cytoplasmic localization of NFκB p65 (yellow) is compared to nuclei (blue, DAPI stain) in mock-infected keratocytes (M). Adenoviral-infected (V) keratocytes demonstrate nuclear localization of NFκB p65 and p50. Nuclear translocation of NFκB p65 was reduced in the presence of inhibitors of p38 (SB) and ERK (PD). The nuclear localization of both NFκB p65 and p50 was partially reduced upon pretreatment with an inhibitor of JNK (SP) and completely blocked with an inhibitor of Src (PP2). Isotype control is shown (Ctrl). Viral infection also induced nuclear translocation of cREL (B), while all signaling inhibitors tested reduced the nuclear translocation of cREL.