Molecular Vision: Štabuc-Šilih, Mol Vis 2009; 15:2848-2860. Table 2

Table 2. Oligonucleotide primers and PCR temperatures used for single-stranded conformational analysis and sequencing of the COL4A4 gene [22].

*COL4A4*	Sense primer (5'→3')	Antisense primer (5'→3')	Length (bp)	Annealing temperature (°C)
EX 2	TCTGGAAGAGAAGACTGGCA	AAGCAGGCAATCACACTGA	153	54
EX 3	TGTTTAAATTAATCTGCGTT	GCAACCAGAGCTAGTG	105	48
EX 4	CGATGAGTACTGGTATACTA	ATGCTGCCCATGTTGGTCTT	152	50
EX 5	ACCCCCATTTCTTTTTAATC	GGTGAGTCTTTCATGTGAAT	208	54
EX 6	TCTCTTTGTTTTATTTTCTG	GATGAGTACTTCTGCCTTTT	127	47
EX 7	TTTCGCAAAAATGCTTCACT	CCACAGGGCCTGTTCACTTA	211	60
EX 8	TACTGAAATGGTAATACGCT	CATGGGCTTACCTATTTGGA	184	48
EX 9	TGTGTGGACTTAAAGCGATG	TAGAGCCTGCTCAGGAGACT	96	53
EX 10	TTGGGTAACAGATGCACTGA	AAGGGATCACATCAGCAGTG	129	55
EX 11	TTGTGTTTTTTTCTCCCTTG	TTTCATTGTTCAGGGCTCTA	109	50
EX 12	AGCCAGAAGTCTTAATTGCT	TCACCATTTGCTCCTCAGAG	156	54
EX 13	GGGTGGAAACCTTCAAAACA	TACTTTCCAAGGTGACATAT	179	50
EX 14	GGAGATGGAATTCAGTATGT	AAAGACCATGAGAAATAACA	197	53
EX 15	CCCCTCTAAATGTTGTCATC	TTTGAGCTTGTGGGACTACT	180	54
EX 16	AATGATGCACTGAGCTGGTT	GCACGCAACAGTACAACTTC	200	53
EX 17	ATTTGTCACCCCGTCACTTT	GAATGATTCCTGGCAATACT	201	50
EX 18	CCAGGCAACATGAGTAAAAT	TGGAGGAACTGAATAGGAAC	155	50
EX 19	TGCACATACCATTTGTTTAT	CCAGGGCACATCAGGGCATC	175	50
EX 20	TTCTTCTACAGAGACGTTT	TGCTAATGGATATGAATAAG	259	52
EX 21	TATAGAAGACAGTCAGAAAA	TAGAAATTCTACCTTTGGTG	181	50
EX 22	AAATATGACAAATCTGCCAT	GGAAAGATGACTGGTAAGAG	227	50
EX 23	TGATCCATCACAATTAACCT	CAGGGAGTTAAGTGATTGAT	149	53
EX 24	ACTTTACCCTCTGCTGATAA	GGGAAATAGTTGTTTGTATG	223	50
EX 25	GACATTCAGTGGTTGGTAAT	TAAACACTTGTACCCCAAAG	280	60
EX 26	TCAGTTATGTGAATGCCGCT	TGGGAAGTATATAAGACAGT	147	50
EX 27	TGAGTCTGTGTTTTGTTTTT	AAAAAAAAAAACCTCAC	210	52
EX 28	ATTGGTTCTATACTTGCACA	TCTATGCACCAAAAGGACAG	309	54
EX 29	TGGGCCATCTGTATAGTTTT	TAATAGTAAGTAGGGTAAGC	269	57
EX 30	GCCTTCACACACTGTGGTCA	ATGGGAGGACATCATGGAAA	240	55
EX 31	TCCTAAAACTTTATGCTCTC	TCAAATACCAGAAACAAATG	221	53
EX 32	CCTGTTCATTTTGTTCTTGC	TGTCAACTTATTTGATATGG	187	57
EX 33	TTTCAGCAGAGACCTGTAAC	AAGAACAGAAAGGTTTTATT	271	52
EX 34	GTTGTGCATGTGCCATTTGT	GATGGCTTCTGTATCTCC	154	50
EX 35	TGAGACCAAATTAAATTGTC	TCATTGCCAGCTAGAAGTAA	210	52
EX 36	CAAACGGCAACTCTGATGTT	AGTGCTCAGGAAGTCTCCAG	183	55
EX 37	TATCTGGCCATCTGCAAAAC	TTGTGGGATGGGCTTCATTT	173	55
EX 38	GCGTTTGTGGCTAGAGTGAG	GAACCATGGACTGAAGCTCAG	190	57
EX 39	AGGCACTATAACAGGGACAAGA	GGAGTAACGTAAACCTTCCA	256	60
EX 40	ACCTTCCAAATGCAATGAGG	CATCCTTTGTCATGATTCTCTC	184	53
EX 41	TTTTTGTCTCTTCTCTGTGG	AGTTATTCACATATTACTTA	218	48
EX 42	GCCCTCATTTTTATGTTTTG	GTTGGAAGCTCACCTGGAAG	153	54
EX 43	GACTGGCCTCGTTTG	TTAATATCCTTACAGCACCC	180	50
EX 44	ATTACACAAGCGGTGATTCC	TGGCTCCTTCTGGTCCTCTC	118	56
EX 45	CACCAGCATCATAAACTT	AGGTTTACAGTGTCAGAGAA	186	53
EX 46	AGTGCCAGAACAGAGGTGCT	GGAGATGGGCGATCCTGTA	297	57
EX 47	ACACCAGCTGTCTCTTCTTC	TGAATGAGCCAGGGTTT	353	57
EX 48	GTGTGTGTCTGAGCCCTAAT	TGGTGAATTTCGCATTCT	322	50