Table 2 of Štabuc-Šilih, Mol Vis 2009; 15:2848-2860.
Table 2. Oligonucleotide primers and PCR temperatures used for single-stranded conformational analysis and sequencing of the COL4A4 gene [22].
COL4A4 |
Sense primer (5'→3') |
Antisense primer (5'→3') |
Length (bp) |
Annealing temperature (°C) |
---|---|---|---|---|
EX 2 | TCTGGAAGAGAAGACTGGCA | AAGCAGGCAATCACACTGA | 153 | 54 |
EX 3 | TGTTTAAATTAATCTGCGTT | GCAACCAGAGCTAGTG | 105 | 48 |
EX 4 | CGATGAGTACTGGTATACTA | ATGCTGCCCATGTTGGTCTT | 152 | 50 |
EX 5 | ACCCCCATTTCTTTTTAATC | GGTGAGTCTTTCATGTGAAT | 208 | 54 |
EX 6 | TCTCTTTGTTTTATTTTCTG | GATGAGTACTTCTGCCTTTT | 127 | 47 |
EX 7 | TTTCGCAAAAATGCTTCACT | CCACAGGGCCTGTTCACTTA | 211 | 60 |
EX 8 | TACTGAAATGGTAATACGCT | CATGGGCTTACCTATTTGGA | 184 | 48 |
EX 9 | TGTGTGGACTTAAAGCGATG | TAGAGCCTGCTCAGGAGACT | 96 | 53 |
EX 10 | TTGGGTAACAGATGCACTGA | AAGGGATCACATCAGCAGTG | 129 | 55 |
EX 11 | TTGTGTTTTTTTCTCCCTTG | TTTCATTGTTCAGGGCTCTA | 109 | 50 |
EX 12 | AGCCAGAAGTCTTAATTGCT | TCACCATTTGCTCCTCAGAG | 156 | 54 |
EX 13 | GGGTGGAAACCTTCAAAACA | TACTTTCCAAGGTGACATAT | 179 | 50 |
EX 14 | GGAGATGGAATTCAGTATGT | AAAGACCATGAGAAATAACA | 197 | 53 |
EX 15 | CCCCTCTAAATGTTGTCATC | TTTGAGCTTGTGGGACTACT | 180 | 54 |
EX 16 | AATGATGCACTGAGCTGGTT | GCACGCAACAGTACAACTTC | 200 | 53 |
EX 17 | ATTTGTCACCCCGTCACTTT | GAATGATTCCTGGCAATACT | 201 | 50 |
EX 18 | CCAGGCAACATGAGTAAAAT | TGGAGGAACTGAATAGGAAC | 155 | 50 |
EX 19 | TGCACATACCATTTGTTTAT | CCAGGGCACATCAGGGCATC | 175 | 50 |
EX 20 | TTCTTCTACAGAGACGTTT | TGCTAATGGATATGAATAAG | 259 | 52 |
EX 21 | TATAGAAGACAGTCAGAAAA | TAGAAATTCTACCTTTGGTG | 181 | 50 |
EX 22 | AAATATGACAAATCTGCCAT | GGAAAGATGACTGGTAAGAG | 227 | 50 |
EX 23 | TGATCCATCACAATTAACCT | CAGGGAGTTAAGTGATTGAT | 149 | 53 |
EX 24 | ACTTTACCCTCTGCTGATAA | GGGAAATAGTTGTTTGTATG | 223 | 50 |
EX 25 | GACATTCAGTGGTTGGTAAT | TAAACACTTGTACCCCAAAG | 280 | 60 |
EX 26 | TCAGTTATGTGAATGCCGCT | TGGGAAGTATATAAGACAGT | 147 | 50 |
EX 27 | TGAGTCTGTGTTTTGTTTTT | AAAAAAAAAAACCTCAC | 210 | 52 |
EX 28 | ATTGGTTCTATACTTGCACA | TCTATGCACCAAAAGGACAG | 309 | 54 |
EX 29 | TGGGCCATCTGTATAGTTTT | TAATAGTAAGTAGGGTAAGC | 269 | 57 |
EX 30 | GCCTTCACACACTGTGGTCA | ATGGGAGGACATCATGGAAA | 240 | 55 |
EX 31 | TCCTAAAACTTTATGCTCTC | TCAAATACCAGAAACAAATG | 221 | 53 |
EX 32 | CCTGTTCATTTTGTTCTTGC | TGTCAACTTATTTGATATGG | 187 | 57 |
EX 33 | TTTCAGCAGAGACCTGTAAC | AAGAACAGAAAGGTTTTATT | 271 | 52 |
EX 34 | GTTGTGCATGTGCCATTTGT | GATGGCTTCTGTATCTCC | 154 | 50 |
EX 35 | TGAGACCAAATTAAATTGTC | TCATTGCCAGCTAGAAGTAA | 210 | 52 |
EX 36 | CAAACGGCAACTCTGATGTT | AGTGCTCAGGAAGTCTCCAG | 183 | 55 |
EX 37 | TATCTGGCCATCTGCAAAAC | TTGTGGGATGGGCTTCATTT | 173 | 55 |
EX 38 | GCGTTTGTGGCTAGAGTGAG | GAACCATGGACTGAAGCTCAG | 190 | 57 |
EX 39 | AGGCACTATAACAGGGACAAGA | GGAGTAACGTAAACCTTCCA | 256 | 60 |
EX 40 | ACCTTCCAAATGCAATGAGG | CATCCTTTGTCATGATTCTCTC | 184 | 53 |
EX 41 | TTTTTGTCTCTTCTCTGTGG | AGTTATTCACATATTACTTA | 218 | 48 |
EX 42 | GCCCTCATTTTTATGTTTTG | GTTGGAAGCTCACCTGGAAG | 153 | 54 |
EX 43 | GACTGGCCTCGTTTG | TTAATATCCTTACAGCACCC | 180 | 50 |
EX 44 | ATTACACAAGCGGTGATTCC | TGGCTCCTTCTGGTCCTCTC | 118 | 56 |
EX 45 | CACCAGCATCATAAACTT | AGGTTTACAGTGTCAGAGAA | 186 | 53 |
EX 46 | AGTGCCAGAACAGAGGTGCT | GGAGATGGGCGATCCTGTA | 297 | 57 |
EX 47 | ACACCAGCTGTCTCTTCTTC | TGAATGAGCCAGGGTTT | 353 | 57 |
EX 48 | GTGTGTGTCTGAGCCCTAAT | TGGTGAATTTCGCATTCT | 322 | 50 |