Molecular Vision: Štabuc-Šilih, Mol Vis 2009; 15:2848-2860. Table 1

Table 1. Oligonucleotide primers and PCR temperatures used for single-stranded conformational analysis and sequencing of the COL4A3 gene [29].

*COL4A3*	Sense primer (5'→3')	Antisense primer (5'→3')	Length (bp)	Annealing temperature (°C)
EX 1	CGGACTCGCCCAGGCTCTGA	GACGCGTGGAGGAGGGATG	176	62
EX 2	AACAAAACCCTTTCTCTT	AAGCAGTATTAGGGTTTGTT	113	49
EX 3	TGTGTGTTTCTCACCTCGT	GATTTTCCAAGCTTGCAG	151	54
EX 4	TTTCTTTTTTCACTTGAATCT	ACGATCAGGGTGGACTG	99	50
EX 5	CCCCCTCCTTTTTCCTATGT	TTTCTAGCTACGGATTTTTC	102	45
EX 6	CCTCATTGAGACTTGTTCT	TCATCTTCTGTGTGAAAAGT	116	42
EX 7	AATAATAAGAAACTTTGTATGT	GGGAATTAGGCATGCAAA	106	49
EX 8	GTTGTTCATAGGTTGCTTTT	TCAGTGACAGCATTCCAC	83	46
EX 9	GATGTTTGATGAACTTCTTC	ATAGGGACCTTCTCTGAA	134	52
EX 10	TACTCTTATTCTTCTCTCAA	CTGTAGCAAGGATGACT	117	49
EX 11	GTGATTTTCATTTGTGGATT	AGCTGTTACATCATATGAACT	93	48
EX 12	AATAATTTGGTTTTGTGTT	CCTGCTAATAAAACATAGTA	100	44
EX 13	ACTCCTGAGTGTTTTTGT	TAATCATAAAATCGCAGA	126	49
EX 14	TTGTAACAATGTTGAACTGT	ATGGGGACAATATAACTTTA	124	50
EX 15	ATAAAATTTGACATGGCTCT	GACTAATCAAAACTGCACAT	133	49
EX 16	TTTCATGTTTTTGATTTGTT	TGACATTTTTACTACCTCCA	116	46
EX 17	GACCCATTTCTTTTTGTTCT	AAAATAGGCTATTAGGGAGA	110	48
EX 18	CACAATTTGTAAATGTCTT	GATATTGTCTTTAATCACAC	94	46
EX 19	TCTGTATTTGTTTCTTTCTC	AAATGCTTTAGGAAGAAAT	141	52
EX 20	TTATATCTTTCTAAGCCATT	CCTTTGTAATAGCATTTCTA	125	47
EX 21	TCTCCATTGTGCAATTTTTA	CTAAGCTGTGAGGAGGGTTT	367	53
EX 22	ATTGTCTTTGGTGCTGTAT	GGCTTATCCTAATACAACAT	156	49
EX 23	AAGTAATGCTAGTATGCTCTC	TGTGCTTGCAAAAACACT	162	49
EX 24	TAGTTAATAATTCGTTGA	AAGATTTAAAAACATGAA	121	44
EX 25	ACAGATTCATTTGTGTACTA	GAGGGTAAAGTTGCTAAATA	234	54
EX 26	ATTCAAACACATTCCTGT	GGACTGGAAAGAAAACTAA	219	51
EX 27	ATCTTATGACCACAAATTTC	CAGATTTGGCAGAGGATA	142	54
EX 28	AGATGCATATGTGTATTTGT	CTTCTAAATATCCACAACAA	182	44
EX 29	CTAATCCTACAACAATGTTT	TTCTGTGATAGCTTGAATTT	164	47
EX 30	ATAGTAATAACACAATTTCT	GAGAAAAGTAATGACACT	209	45
EX 31	CCTGGGTATATACTTGTGCT	ATGTCTCCTGCCCTTCTGG	191	52
EX 32	GGAAAGCATTTGTGGGTTA	ACAGAGCCACCTTAAGAAGA	276	52
EX 33	TGCTTTGTGTTAATTTGTTT	TCCTGCTATTTAGAAAGACA	149	52
EX 34	AAGGACCTGATGTTGTTACT	TCTGATGTCCTGATTCCA	202	52
EX 35	TTCTTGTTAATACCTGGTTT	TGATATTTTTCTATTTGAGA	160	49
EX 36	CAGGGCAATAACTACTTA	GCTCATAACAGGACCTTA	146	50
EX 37	TACTCTATGTTTTCCCCCTA	TCCACCACTAAAATGTAAAT	206	52
EX 38	TATGAGAATTTTAAAGGTAT	TCCAGCTTTTAGAATTGTAA	195	48
EX 39	GGTGATCTTTTTTCTTCCTT	CCCACATGAAAAGGAAAAAG	160	50
EX 40	GGGGTTTTGGGTTTTTTT	ACGGATCAAAGATAATGAGCA	156	52
EX 41	CAATTATTAACATGCCAAGA	TACATTAGGACAGGGAAGAA	112	50
EX 42	AAAGAAACTTATTAAGCCTT	TTGTTATTTTATGCTGTTTA	253	52
EX 43	ATACTGACAGACTTTTCAT	TAATAATGAGTCAAAATAAT	191	50
EX 44	GTTTTGCTCCCTTTATTTGA	ATATAAAGAGCAATGCACAA	129	51
EX 45	GGAAACCCATTGATCTAAGT	ACCTTTCTTCATTGACAGCA	164	51
EX 46	TGAGGCCATCATCTTCTTCT	TCCTAGTGATCCAAGTCAAT	199	50
EX 47	CCACCTTACTTTTCATCCTAT	ACTTCTTCGGTGAGGAAAC	190	59
EX 48	CTTTGAAAAAACGAGTTTAAG	TTACAATCTGCATGTGGAA	324	61
EX 49	CTAGTAACGATGCTGAAAATAAC	TCACTTGGTCCCATTGTAA	284	54
EX 50	TTCCCTTGTAATGGAATGAAA	CACATTTTACCCAGCACAAT	271	52
EX 51	AACCCCAATGGACAGAGTGTT	TGAATAGTTCTGCAATTGAGT	272	63
EX 52	CAGCAAAAATTCCCTTTTATG	TGTTCTTTAGGATGAAAAAT	190	47