Table 1 of Štabuc-Šilih, Mol Vis 2009; 15:2848-2860.
Table 1. Oligonucleotide primers and PCR temperatures used for single-stranded conformational analysis and sequencing of the COL4A3 gene [29].
COL4A3 |
Sense primer (5'→3') |
Antisense primer (5'→3') |
Length (bp) |
Annealing temperature (°C) |
---|---|---|---|---|
EX 1 | CGGACTCGCCCAGGCTCTGA | GACGCGTGGAGGAGGGATG | 176 | 62 |
EX 2 | AACAAAACCCTTTCTCTT | AAGCAGTATTAGGGTTTGTT | 113 | 49 |
EX 3 | TGTGTGTTTCTCACCTCGT | GATTTTCCAAGCTTGCAG | 151 | 54 |
EX 4 | TTTCTTTTTTCACTTGAATCT | ACGATCAGGGTGGACTG | 99 | 50 |
EX 5 | CCCCCTCCTTTTTCCTATGT | TTTCTAGCTACGGATTTTTC | 102 | 45 |
EX 6 | CCTCATTGAGACTTGTTCT | TCATCTTCTGTGTGAAAAGT | 116 | 42 |
EX 7 | AATAATAAGAAACTTTGTATGT | GGGAATTAGGCATGCAAA | 106 | 49 |
EX 8 | GTTGTTCATAGGTTGCTTTT | TCAGTGACAGCATTCCAC | 83 | 46 |
EX 9 | GATGTTTGATGAACTTCTTC | ATAGGGACCTTCTCTGAA | 134 | 52 |
EX 10 | TACTCTTATTCTTCTCTCAA | CTGTAGCAAGGATGACT | 117 | 49 |
EX 11 | GTGATTTTCATTTGTGGATT | AGCTGTTACATCATATGAACT | 93 | 48 |
EX 12 | AATAATTTGGTTTTGTGTT | CCTGCTAATAAAACATAGTA | 100 | 44 |
EX 13 | ACTCCTGAGTGTTTTTGT | TAATCATAAAATCGCAGA | 126 | 49 |
EX 14 | TTGTAACAATGTTGAACTGT | ATGGGGACAATATAACTTTA | 124 | 50 |
EX 15 | ATAAAATTTGACATGGCTCT | GACTAATCAAAACTGCACAT | 133 | 49 |
EX 16 | TTTCATGTTTTTGATTTGTT | TGACATTTTTACTACCTCCA | 116 | 46 |
EX 17 | GACCCATTTCTTTTTGTTCT | AAAATAGGCTATTAGGGAGA | 110 | 48 |
EX 18 | CACAATTTGTAAATGTCTT | GATATTGTCTTTAATCACAC | 94 | 46 |
EX 19 | TCTGTATTTGTTTCTTTCTC | AAATGCTTTAGGAAGAAAT | 141 | 52 |
EX 20 | TTATATCTTTCTAAGCCATT | CCTTTGTAATAGCATTTCTA | 125 | 47 |
EX 21 | TCTCCATTGTGCAATTTTTA | CTAAGCTGTGAGGAGGGTTT | 367 | 53 |
EX 22 | ATTGTCTTTGGTGCTGTAT | GGCTTATCCTAATACAACAT | 156 | 49 |
EX 23 | AAGTAATGCTAGTATGCTCTC | TGTGCTTGCAAAAACACT | 162 | 49 |
EX 24 | TAGTTAATAATTCGTTGA | AAGATTTAAAAACATGAA | 121 | 44 |
EX 25 | ACAGATTCATTTGTGTACTA | GAGGGTAAAGTTGCTAAATA | 234 | 54 |
EX 26 | ATTCAAACACATTCCTGT | GGACTGGAAAGAAAACTAA | 219 | 51 |
EX 27 | ATCTTATGACCACAAATTTC | CAGATTTGGCAGAGGATA | 142 | 54 |
EX 28 | AGATGCATATGTGTATTTGT | CTTCTAAATATCCACAACAA | 182 | 44 |
EX 29 | CTAATCCTACAACAATGTTT | TTCTGTGATAGCTTGAATTT | 164 | 47 |
EX 30 | ATAGTAATAACACAATTTCT | GAGAAAAGTAATGACACT | 209 | 45 |
EX 31 | CCTGGGTATATACTTGTGCT | ATGTCTCCTGCCCTTCTGG | 191 | 52 |
EX 32 | GGAAAGCATTTGTGGGTTA | ACAGAGCCACCTTAAGAAGA | 276 | 52 |
EX 33 | TGCTTTGTGTTAATTTGTTT | TCCTGCTATTTAGAAAGACA | 149 | 52 |
EX 34 | AAGGACCTGATGTTGTTACT | TCTGATGTCCTGATTCCA | 202 | 52 |
EX 35 | TTCTTGTTAATACCTGGTTT | TGATATTTTTCTATTTGAGA | 160 | 49 |
EX 36 | CAGGGCAATAACTACTTA | GCTCATAACAGGACCTTA | 146 | 50 |
EX 37 | TACTCTATGTTTTCCCCCTA | TCCACCACTAAAATGTAAAT | 206 | 52 |
EX 38 | TATGAGAATTTTAAAGGTAT | TCCAGCTTTTAGAATTGTAA | 195 | 48 |
EX 39 | GGTGATCTTTTTTCTTCCTT | CCCACATGAAAAGGAAAAAG | 160 | 50 |
EX 40 | GGGGTTTTGGGTTTTTTT | ACGGATCAAAGATAATGAGCA | 156 | 52 |
EX 41 | CAATTATTAACATGCCAAGA | TACATTAGGACAGGGAAGAA | 112 | 50 |
EX 42 | AAAGAAACTTATTAAGCCTT | TTGTTATTTTATGCTGTTTA | 253 | 52 |
EX 43 | ATACTGACAGACTTTTCAT | TAATAATGAGTCAAAATAAT | 191 | 50 |
EX 44 | GTTTTGCTCCCTTTATTTGA | ATATAAAGAGCAATGCACAA | 129 | 51 |
EX 45 | GGAAACCCATTGATCTAAGT | ACCTTTCTTCATTGACAGCA | 164 | 51 |
EX 46 | TGAGGCCATCATCTTCTTCT | TCCTAGTGATCCAAGTCAAT | 199 | 50 |
EX 47 | CCACCTTACTTTTCATCCTAT | ACTTCTTCGGTGAGGAAAC | 190 | 59 |
EX 48 | CTTTGAAAAAACGAGTTTAAG | TTACAATCTGCATGTGGAA | 324 | 61 |
EX 49 | CTAGTAACGATGCTGAAAATAAC | TCACTTGGTCCCATTGTAA | 284 | 54 |
EX 50 | TTCCCTTGTAATGGAATGAAA | CACATTTTACCCAGCACAAT | 271 | 52 |
EX 51 | AACCCCAATGGACAGAGTGTT | TGAATAGTTCTGCAATTGAGT | 272 | 63 |
EX 52 | CAGCAAAAATTCCCTTTTATG | TGTTCTTTAGGATGAAAAAT | 190 | 47 |