Molecular Vision: Crosson, Mol Vis 2009; 15:296-311. Table 2

Table 2. Gene Ontology categories significantly altered during hypoxia and recovery

Hypoxia	p value	Recovery	p value
Ontological Category	p value	Ontological Category	p value
		Biologic Process
Biologic Process		Synaptic Vesicle Endocytosis	0.005
Translation	0.002	Sulfur Metabolism	0.014
Regulation of Translation	0.003	Microtubule Polymerization	0.016
Carboxylic Acid Metabolism	0.014	Cytoskeleton-Dependent Intracellular Transport	0.017
Regulation of Heart Contraction	0.022	Microtubule-Based Movement	0.017
Nitrogen Compound Metabolism	0.023	Oxygen and Reactive Oxygen Species Metabolism	0.018
Carbohydrate Transport	0.026	Regulation of Heart Contraction	0.026
Monosaccharide Metabolism	0.041	DNA Damage Response, Signal Transduction	0.029
Transport	0.041	Microtubule Polymerization or Depolymerization	0.029
		Microtubule-Based Process	0.034
Cellular Component		Protein Polymerization	0.034
Membrane-Bound Vesicle	0.003	Microtubule Cytoskeleton Organization and Biogenesis	0.046
Vesicle	0.003	Response to Oxidative Stress	0.049
Golgi Vesicle	0.004
Cytoplasmic Membrane-Bound Vesicle	0.005	Cellular Component
Chromatin	0.007	Vesicle Membrane	0.002
Nuclear Chromosome	0.007	Intracellular Non-Membrane-Bound Organelle	0.003
Chromosome	0.008	Condensed Chromosome	0.016
Intracellular Non-Membrane-Bound Organelle	0.013	Integral to Membrane of Membrane Fraction	0.016
Condensed Chromosome	0.014	Tubulin	0.016
Integral-to-Membrane of Membrane Fraction	0.014	Voltage-Gated Calcium Channel Complex	0.023
Perinuclear Region	0.026	Cytoskeleton	0.034
Mitochondrial Membrane	0.03	Organelle Membrane	0.035
Organelle Envelope	0.034	Cytosol	0.043
Clathrin-coated Vesicle	0.043	Microtubule	0.046
Envelope	0.043	Protein Complex	0.047
Vesicle Membrane	0.043
		Molecular Function
Molecular Function		Amino Acid Binding	0.034
Amino Acid Binding	0.032	Calmodulin Binding	0.041
Cation:Amino Acid Symporter Activity	0.032	Voltage-Gated Calcium Channel Activity	0.049
Sugar Transporter Activity	0.032	Calcium Ion Binding	0.05
Structural Molecule Activity	0.048

The genes identified as differentially expressed when comparing hypoxic and control retinal samples and when comparing recovery and control retinal samples were examined for their biologic association to GO categories (detailed in the text). Using GOMiner software, three independent category structures (biologic process, cellular component, and molecular function) were initially constructed and the genes identified in this study were examined for their distribution within these three GO category structures. The significance of the calculated enrichment in each GO category was calculated as a p-value using Fisher's Exact Test. A p<0.05 was considered significant.