Figure 2 of Crosson, Mol Vis 2009; 15:296-311.


Figure 2. qRT–PCR analysis of known hypoxia-associated mRNAs. For each mRNA, transcript abundance, normalized to acidic ribosomal protein P0, was calculated by qRT–PCR. Values were then further normalized to the control level of each transcript. Data represent mean (±SD) of 5 independent experiments. A: The expression of VEGF and VEGF receptors Flk-1 and Flt-1 mRNA was measured in the rat retina immediately following 3 h of hypoxia and after 24 h of recovery in air. VEGF and Flk-1 were significantly higher in hypoxic samples as compared to control. Flt-1 tended to increase during hypoxia, but the difference from control was not significant. B: The expression of erythropoietin (EPO) and erythropoietin receptor (EPOR) mRNA was measured in the rat retina immediately following 3 h of hypoxia and after 24 h of recovery in air. EPO mRNA levels were significantly higher in hypoxic samples as compared to control. A significant difference was also observed for the EPOR control and hypoxia mRNA levels between hypoxic and recovery samples. The asterisks indicate significance levels assessed via ANOVA followed by post-hoc tests: * - p<0.05; ** - p<0.01; *** - p<0.001.