Table 1 of Gong, Mol Vis 2009; 15:2829-2840. Table 1 of Gong, Mol Vis 2009; 15:2829-2840.
Table 1. Sense oligonucleotides for site-directed mutagenesis in CRYAA.
| Mutation | Oligonucleotides (sense, 5’ to 3’; bold underlined letters indicate specific base change) | Expression constructs |
|---|---|---|
| R12C (c.34C>T) | ATCCAGCACCCCTGGTTCAAGTGCACCCTGGGGCCCTTCTAC | pHis/myc-CRYAAR12C |
| R21L (c.62C>T) | TGGGGCCCTTCTACCCCAGCCTGCTGTTCGACCAGTTTTTCG | pHis/myc-CRYAAR21L |
| R49C (c.145C>T) | TCCACCATCAGCCCCTACTACTGCCAGTCCCTCTTCCGCACCG | pHis/myc-CRYAAR49C |
| G98R (c.292G>A) | GTGGAGATCCACAGAAAGCACAACGAG | pHis/myc-CRYAAG98R |
| R116C (c.346C>T) | ACATTTCCCGTGAGTTCCACTGCCGCTACCGCCTGCCGTCC | pHis/myc-CRYAAR116C |