Figure 3. Trimethylamine N-oxide reduced the detergent insolubility of C98R αA-crystallin (CRYAA). A: Western blot analysis of myc (CRYAA) showed that the WT was mainly Tx-soluble and not affected by 4-PBA or TMAO treatment.
Untreated G98R CRYAA was predominantly Tx insoluble, which was significantly reduced after 4-PBA (1 mM) or TMAO (100 mM) treatment
for 2 days. The asterisk indicates a p<0.05 by independent Student t test. DMSO (1%) did not affect mutant insolubility. B: TMAO reduced Tx-insoluble G98R CRYAA dose dependantly. Treatment with TMAO (50 mM or higher) for 2 days significantly decreased
Tx-insoluble G98R CRYAA. The asterisk indicates a p<0.05 by independent Student t test. C: TMAO (100 mM) reduced Tx-insoluble G98R CRYAA in a time-dependant manner. WT: wild-type; Tx: Triton X-100; 4-PBA: 4-phenylbutyric
acid; TMAO: trimethylamine N-oxide; DMSO: dimethylsulfoxide.