Figure 4. Distribution of intravitreally injected bevacizumab in the FcRn knockout and wild-type mice. A and B show the distribution of intravitreally administered bevacizumab (green) around retinal blood vessels in an FcRn knockout mouse and wild-type mouse 5 h post injection, respectively. C shows the normalized fluorescent intensity across the retinal vascular endothelium in a wild-type mouse. Arrows in B indicate the direction over which the fluorescence profiles were obtained. The normalized fluorescence intensity profile in panel C shows a fluorescence intensity gradient across the retinal vascular endothelium from the abluminal to luminal sides, indicating the penetration of bevacizumab into the blood system in the wild-type mouse. A, B: Cell nuclei were stained with DAPI (blue).