Figure 1. The distribution in rat eyes of intravitreally administered fluorescent dye labeled full-length IgG (red). A-C show the distribution of the full-length IgG in the normal retina at 30 min, 6 h, and 15 h post intravitreal administration, respectively. D and E show the distribution of the full-length IgG in the laser-photocoagulated retina at 1 h and 3 h post intravitreal administration, respectively. Intravitreally administered full-length antibody overcame the inner limiting membrane barrier and diffused into the deeper retinal structures in both laser photocoagulated and control rat retinas. A–E: Cell nuclei were stained with DAPI (blue). Abbreviations: inner limiting membrane (ILM), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), external limiting membrane (ELM), and Bruch’s membrane (BM).