Figure 3. Fluorescence microscopy ND4FLAG.
Confocal microscopy of a retinal flat mount from an eye injected with
the scAAV-ND4FLAG packaged in VP3 capsids with the tyrosine to
phenylalanine transition at position 444 revealed many cells were
labeled with the anti-FLAG antibody (A). RGCs expressing Thy1.2 (B)
with ND4FLAG expressing cells are shown (C).
Confocal imaging of longitudinal cross-sections of the retina revealed
ND4FLAG immunofluorescence within the RGC layer (D).
Immunofluorescent labeling of Thy1.2 positive cells with cy2 was
somewhat fading by the time of confocal imaging of cryosectioned retina
(E). A merged panel counterstained with DAPI shows ND4FLAG
immunofluorescence within the RGC layer (F). Abbreviations: INL
represents inner nuclear layer, RGC represents retinal ganglion cell.
Scale bar equals to 50 µm.