Figure 3. This shows the effect of RSK2 siRNA on proliferation of fibroblasts. A: NIH3T3 cells were transfected with RSK2 siRNAs and cultured for 24 h. After that, cells were treated with or without 0.33 μg/ml bFGF for 24 h. Cells were lysed and the whole cell extract was loaded onto 7.0% polyacrylamide gel (SDS-PAGE). Endogenous RSK2 expression was blotted with an anti-RSK2 antibody as indicated. Blots with an anti-α tubulin antibody were also performed to standardize the total protein volume loaded. B: The number of living cells was estimated by MTS assays and three independent experiments were performed. The results were statistically analyzed and the ratio of surviving cells (viable) is shown as the mean±standard error of the means. (The asterisk indicates a p<0.05, Student’s t test).