Table 1 of Carr, Mol Vis 2009; 15:283-295.

Table 1. PCR primer sequences

mRNA Forward primer (5′−3′) Reverse Primer (5′−3′) Tm Amplicon
size		 Accession
No
Itgav CCTGTGCCTGTGTGGGTGAT GGTGGCCGGACCCGTTTA 58 110 NM_002210
Itgb5 CGAGCGTGGGCACTGTCTCT GCAGGCACTCGACGCAATCT 58 128 NM_002213
CD36 TGCAGCCTCATTTCCACCTT CAAAGGCCTTGGATGGAAGA 54 151 NM_001001547
CD81 TCTGGAGCTGGGAGACAAGC GGATGACCAGGCAGGTGAAG 58 163 NM_004356
Ctsd AGCTGGGAGGCAAAGGCTAC CCCTGTTGTTGTCACGGTCA 56 188 NM_001909
Cltc CGTCACTGCACTCCATCCTG ACCCACAGGGTCTCCACAGA 58 270 NM_004859
Clta AGAGCCACCCTGTGGAAACA GCTTCCCTCCCCTTCCTCTT 56 215 NM_001833
Cltb AACGGTCCTGCTGATGGCTA CACTCTGTGCCTGGGGTCTC 56 280 NM_001834
Fak ATGTGACGGGCCTGGTGAAAGG TGGGTGCTGGCTGGTAGGAG 58 160 AY323812
Gas6 GCGGAATCTGGTCATCAAGG TCAGCCAGTTCCAGCTCCTC 56 199 NM_000820
MerTK GTGAGGCAGCGTGCATGAAAG GGGCTTTGGGATGCCTTGAG 58 95 NM_006343
Mfge8 CTTGGCTTCTCAGCCCCTTT GTGAGGACTGGGGGTTAGGG 56 209 NM_005928
ProS1 CGGAAAATGGATTGCTGGAA ACCAGAAACCAAGGCAAGCA 52 340 NM_000313
Tbp GAACCACGGCACTGATTTTC CCCCACCATATTCTGAATCT 52 157 NM_003194

Primers were designed to amplify human sequences for alphaV integrin (Itgav), β5 integrin (Itgb5), CD36 molecule (CD36), CD81 molecule (CD81), Cathepsin D (Ctsd), Clatherin - heavy chain (Cltc), and light chains, (Clta and Cltb), Focal Adhesion Kinase (Fak), Growth arrest specific gene 6 (Gas6), Mer Tyrosinse Kinase (MerTK), Milk fat globule-EGF factor 8 protein (Mfge8), Protein S (ProS1), and TATA box binding protein (Tbp).

Carr, Mol Vis 2009; 15:283-295. http://www.molvis.org/molvis/v15/a29

©2009 Molecular Vision http://www.molvis.org/molvis/

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