Molecular Vision: Gao, Mol Vis 2009; 15:2683-2695. Table 1

Table 1. Primers and PCR conditions of cell cycle regulator genes

Name	Sequence	Product size (bp)	Tm (°C)
CDK1	F: TTTTCAGAGCTTTGGGCACT	195	55
CDK1	R: CCATTTTGCCAGAAATTCGT	195	55
CDK2	F: CATTCCTCTTCCCCTCATCA	173	57
CDK2	R: CAGGGACTCCAAAAGCTCTG	173	57
CDK3	F: TTTGCAGAGATGGTGACTCG	167	57
CDK3	R: AGTCCCTTCCTGGTCCACTT	167	57
CDK4	F: GAAACTCTGAAGCCGACCAG	213	57
CDK4	R: AGGCAGAGATTCGCTTGTGT	213	57
Cyclin A	F: TTATTGCTGGAGCTGCCTTT	224	55
Cyclin A	R: CTCTGGTGGGTTGAGGAGAG	224	55
Cyclin A1	F: ACCCCAAGAGTGGAGTTGTG	198	55
Cyclin A1	R: GGAAGGCATTTTCTGATCCA	198	55
Cyclin B1	F: CGGGAAGTCACTGGAAACAT	177	55
Cyclin B1	R: AAACATGGCAGTGACACCAA	177	55
Cyclin B2	F: TTGCAGTCCATAAACCCACA	218	55
Cyclin B2	R: GAAGCCAAGAGCAGAGCAGT	218	55
Cyclin C	F: AGGCCCCACTCTTATGTCCT	231	59
Cyclin C	R: TGGTGAAACCCCGTCTCTAC	231	59
Cyclin D1	F: AACTACCTGGACCGCTTCCT	204	57
Cyclin D1	R: CCACTTGAGCTTGTTCACCA	204	57
Cyclin D2	F: TGGGGAAGTTGAAGTGGAAC	175	57
Cyclin D2	R: ATCATCGACGGTGGGTACAT	175	57
Cyclin D3	F: TGGATGCTGGAGGTATGTGA	190	55
Cyclin D3	R: TGCACAGTTTTTCGATGGTC	190	55
Cyclin E1	F: CAGATTGCAGAGCTGTTGGA	225	57
Cyclin E1	R: TCCCCGTCTCCCTTATAACC	225	57
Cyclin E2	F: CAGGTTTGGAGTGGGACAGT	199	59
Cyclin E2	R: CTCCATTGCACACTGGTGAC	199	59
P16	F: CTCTGGAGGACGAAGTTTGC	158	57
P16	R: CATTCCTCTTCCTTGGTTTCC	158	57
P18	F: TGCACAAAATGGATTTGGAA	223	51
P18	R: GGGCAGGTTCCCTTCATTAT	223	51
P19	F: CTGCAGGTCATGATGTTTGG	229	57
P19	R: CAGCAGTGTGACCCTCTTGA	229	57
P27	F: ATGTCAAACGTGCGAGTGTC	152	57
P27	R: TCTCTGCAGTGCTTCTCCAA	152	57
P21	F: GACACCACTGGAGGGTGACT	172	59
P21	R: CAGGTCCACATGGTCTTCCT	172	59
P107	F: CCAGTGGTGTGGTCAATCAG	164	59
P107	R: GAACAGCGAGTTTGAGGAG	164	59
Rb	F: GGAAGCAACCCTCCTAAACC	153	57
Rb	R: TTTCTGCTTTTGCATTCGTG	153	57
GAPDH	F: ACCCAGAAGACTGTGGATGG	415	55
GAPDH	R: TGCTGTAGCCAAATTCGTTG	415	55