Figure 1 of Gao, Mol Vis 2009; 15:2683-2695.


Figure 1. PKC activation with PMA affects cell cycle in human RPE cells. Flow cytometry analysis of PMA-treated RPE cells (A) shows decreased S phase and increased G2-M phases cell numbers when compared with that of untreated RPE cells (B). For each side scatter plot, the y-axis is the number of cells, while the x-axis is the DNA content. Values from each scatter plot are graphed below panels A and B. Similar results after PMA treatment were obtained in eight independent experiments. C: Immunofluorescence of cellular DNA stained with propidium iodide (PI) showed cells in interphase or at different stages of mitosis. RPE cells were grown on glass coverslips for 24 h, treated either with PMA or with thymeleatoxin for 9 h, and then fixed.