Figure 5 of Izuta, Mol Vis 2009; 15:2663-2672.


Figure 5. Effects of EC-SOD against VEGF-induced cell proliferation and migration in HUVECs and HRMVECs. In the proliferation assay, (A) HUVECs and (B) HRMVECs were incubated with 10 ng/ml VEGF with or without 100 ng/ml EC-SOD for 72 h. EC-SOD significantly suppressed VEGF-induced proliferation in HUVECs and HRMVECs. Data represent means and standard error. The number of Control group (n=12), EC-SOD alone (n=12), VEGF alone (n=18), and VEGF plus EC-SOD (n=18). The value of “Control” defines as 100%. Double sharp (##) denotes p<0.01 versus Control based on the Tukey test. Asterisk (*) and double asterisk (**) denotes p<0.05 and p<0.01 versus VEGF alone based on the Tukey test. C, D: HUVECs and (E) HRMVECs migration were assessed using a wound-healing assay. Briefly, 90% confluent monolayer cells were scratch-wounded, then incubated for 24 h. Images of this type of wounded monolayer are shown for 0 h and 24 h after treatment with 10 ng/ml VEGF with or without 100 ng/ml EC-SOD. C: These pictures indicated the migration cells after stimulated VEGF with or without EC-SOD in HUVECs. Scale bar represents 250 µm. Horizontal lines indicate wound edges. D, E: EC-SOD had no effect by itself, and no effect on VEGF-induced migration (versus VEGF alone) in HUVECs and HRMVECs. Data represent means and standard error (n=4). Double sharp (##) denotes p<0.01 versus Control based on the Tukey test.