Table 2 of Kozulin, Mol Vis 2009; 15:2649-2662.
Antibody | Dilution | Manufacturer |
---|---|---|
Primary antibodies | ||
Anti-mouse ephrin-A1 (goat, poly) | 1:2,500** | R&D Systems |
Anti-human ephrin-A4 (goat, poly) | 1:2,500** | R&D Systems |
Anti Paired Box-2 (Pax2; rabbit, poly)* | 1:100 | Covance |
Control antibody | ||
Normal goat IgG | 1:25,000** | R&D Systems |
Secondary antibodies | ||
Biotinylated horse anti-goat (0.3% PBSR) | 1:200 | Zymed |
Biotinylated goat anti-rabbit (0.3% PBSR) | 1:200 | Zymed |
Visualization | ||
Conjugated goat anti-rabbit Alexa 594 (0.3% PBSR) | 1:1,000 | Invitrogen |
Streptavidin Alexa 488 | 1:1,000 | Invitrogen |