Table 2 of Kozulin, Mol Vis 2009; 15:2649-2662.


Table 2. Antibodies and immunoreagents.

Antibody Dilution Manufacturer
Primary antibodies
Anti-mouse ephrin-A1 (goat, poly) 1:2,500** R&D Systems
Anti-human ephrin-A4 (goat, poly) 1:2,500** R&D Systems
Anti Paired Box-2 (Pax2; rabbit, poly)* 1:100 Covance
Control antibody

Normal goat IgG 1:25,000** R&D Systems
Secondary antibodies
Biotinylated horse anti-goat (0.3% PBSR) 1:200 Zymed
Biotinylated goat anti-rabbit (0.3% PBSR) 1:200 Zymed
Visualization
Conjugated goat anti-rabbit Alexa 594 (0.3% PBSR) 1:1,000 Invitrogen
Streptavidin Alexa 488 1:1,000 Invitrogen