Molecular Vision: Kozulin, Mol Vis 2009; 15:2649-2662. Table 2

Table 2. Antibodies and immunoreagents.

Antibody	Dilution	Manufacturer
Primary antibodies
Anti-mouse ephrin-A1 (goat, poly)	1:2,500**	R&D Systems
Anti-human ephrin-A4 (goat, poly)	1:2,500**	R&D Systems
Anti Paired Box-2 (Pax2; rabbit, poly)*	1:100	Covance
Control antibody
Normal goat IgG	1:25,000**	R&D Systems
Secondary antibodies
Biotinylated horse anti-goat (0.3% PBSR)	1:200	Zymed
Biotinylated goat anti-rabbit (0.3% PBSR)	1:200	Zymed
Visualization
Conjugated goat anti-rabbit Alexa 594 (0.3% PBSR)	1:1,000	Invitrogen
Streptavidin Alexa 488	1:1,000	Invitrogen

Antibodies raised against the two ligands to Eph-A6, ephrin-A1, and -A4, were used to localize these proteins in sections of fetal macaque retina. Anti-Pax2 double immunolabel was used to localize immature astrocytes, while background labeling was visualized using normal goat IgG antibody. *Pax2 is cross-reactive to human and other species. **IgG concentration applied to tissue is equal to ephrin antibodies given the reconstituted antibodies stocks were 0.1 mg/ml for ephrins and 1 mg/ml for IgG.