Figure 8 of Valamanesh, Mol Vis 2009; 15:2634-2648.


Figure 8. Evaluation of autophagy by microtubules-associated protein light chain 3 staining. No intracytoplasmic vacuoles were stained with anti-microtubules-associated protein light chain 3 (MAP-LC3) in control BRECs (A-C). This is also the case for BRECs exposed to 0.1 mg/ml TA for five days (D-F). In the amino acid (AA) deprived medium (positive control), BRECs show multiple MAP-LC3 positive cytoplasmic vacuoles (G-I, arrows in I). Nuclei were stained with DAPI (A, D, G). Scale bar represents 50 µm.