Figure 6. L-DNase II activation in TA treated BRECs. LEI/L-DNase II immuno-labeling was performed in control cells treated with the TA vehicle (1% ethanol). A-C: A cytoplasmic labeling is seen. In cells treated with 0.1 mg/ml TA for 72h (D-F) or 5 days (G-I), cells with condensed nuclei (arrows) present a nuclear staining for LEI/L-DNase II, indicating L-DNase II activation. Nuclei were stained with DAPI (A, D, G). Scale bar represents 50 µm.