Figure 2. Absence of TUNEL-positive apoptosis in BRECs treated with triamcinolone acetonide. Control cells were treated with the TA vehicle,1% ethanol, (A-C). No TUNEL-positive cells could be observed after exposure to 0.1 mg/ml TA for 72 h (D-F) or five days (D5, G-I). As a positive control for the TUNEL technique, BRECs were treated with 1 μM staurosporine (J-L). Nuclei were stained with DAPI (A, D, G, J). Scale bar represents 60 µm.