Figure 3. Mutational analysis of
CRYGC.
A: Sequence chromatograms of the wild type
CRYGC allele show that the wild type gene encodes a tryptophan residue (TGG) at position 157.
B: Sequence chromatograms of the mutant allele show a c.470G>A transversion that substituted a termination codon (TAG) for
the tryptophan residue at position 157 (W157X).
C: Exon organization and mutational profile of
CRYGC are shown. Codons and the corresponding Greek key motifs are numbered above each exon. The relative locations of the W157X
mutation and three other mutations associated with cataracts in humans are indicated. Mutations are numbered according to
their amino acid position in the processed CRYGC protein.
D: Multiple sequence alignments of
CRYGC with the corresponding segments in human, mouse, and rat is exhibited together with human
CRYGA,
CRYGB, and
CRYGD (sequences found using
ClustalW2). The arrow indicates the W157X mutated position.