Figure 4. The trifluoroethanol (TFE) effects on the protein conformations of transforming growth factor beta-induced protein (TGFBIp) were studied by A: the intrinsic tryptophan fluorescence spectra, B: the 1 anilino-batgakebe-8-sulfonic acid (ANS) fluorescence spectra, and C: the far-ultraviolet circular dichroism (far-UV CD) spectra of recombinant TGFBIp at various TFE concentrations. D: Thioflavin T (ThT) fluorescence intensity was used to determine the fibril formation of TGFBIp at various TFE concentrations (n=3, bar=S.D.). Inset: the fibril formation of TGFBIp at various TFE concentrations over a 24 h period. E: Far-UV CD spectra were obtained by incubating TGFBIp in 10% TFE for 0 and 2 h. Inset: The dot blot experiment of TGFBIp probed by the anti-oligomer antibody.