Figure 3. Acidic pH promotes the fibril formation and fragmentation of transforming growth factor beta-induced protein (TGFBIp). A: Thioflavin T (ThT) fluorescence intensity at 485 nm was used to determine the fibril formation of TGFBIp at various pH conditions (n=5, bar=S.D.). Inset: the intrinsic tryptophan fluorescence traces of TGFBIp at pH 3, 7, and 13. Inset: Formations of the fibrillar oligomeric precursor of TGFBIp at various TFE concentrations were detected by dot blot experiments. B: Chemical fragmentations of TGFBIp under neutral (pH 7) and acidic (pH 3) conditions either at 37 ^oC or 60 ^oC, analyzed on a 15% SDS-PAGE gel.