Figure 4. BOL-303242-X demonstrates similar activity as triamcinolone acetonide (TA) in reducing LPS-induced IL-1ß and ICAM-1 levels in human retinal endothelial cell lysates. Cells were pretreated with BOL-303242-X or TA for 2 h, and then further treated with vehicle (0.1% DMSO), LPS, BOL-303242-X, TA, or combinations of them at the indicated doses for 18 h. Cytokine and ICAM-1 content in the cell lysates was determined by Luminex, and protein content in the lysates by the Micro BCA assay. Data are means±SEM; n=3. The asterisk indicates a p≤0.05 versus the control, and the dagger indicates a p≤0.05 versus LPS. Statistical analysis was performed using one-way ANOVA followed by the Tukey-Kramer test on raw data.