Figure 2. BOL-303242-X demonstrates similar activity as dexamethasone (DEX) in reducing IL-1ß-induced cytokine release from human corneal epithelial cells. Cells were pretreated with BOL-303242-X or DEX for 2 h, and then further treated with vehicle (0.1% DMSO), IL-1ß, BOL-303242-X, DEX, or combinations of them for 18 h. Cytokine content in the conditioned media was determined by Luminex. Data are means±SEM; n=3. The asterisk indicates a p≤0.05 versus IL-1ß, and the dagger indicates a p≤0.05 versus BOL-303242-X at the same concentration. Statistical analysis was performed using two-way ANOVA followed by the Tukey-Kramer test on raw data. A Student’s t-test was used to determine whether IL-1ß was effective. The solid bar is statistically significant versus the gray bar at increasing cytokine release.