Figure 5 of Salinas-Navarro, Mol Vis 2009; 15:2578-2598.


Figure 5. Populations of retinal ganglion cells labeled with 10% hydroxystilbamidine methanesulfonate at 8, 17, 35, and 63 days after lasering. Histograms represent the mean (±SD) total numbers of retinal ganglion cells (RGCs) labeled with 10% hydroxystilbamidine methanesulfonate (OHSt) in the left eye (LE) and right eye (RE) for groups I, II, III, and IV, analyzed 8, 17, 35 or 63 days, respectively, after laser treatment of the LE. To identify RGCs capable of retrograde axonal transport, OHSt was applied to both superior colliculi 1 week before sacrifice. Retinas were imaged with adjacent, nonoverlapping frames captured in a raster pattern. OHSt-labeled RGCs were counted in each frame using image analysis software. The average total numbers of OHSt-labeled RGCs in the LE were approximately one fourth of the total numbers of OHSt-labeled RGCs found in their untreated fellow retinas (RE) at all time points studied. The mean total numbers of OHSt-labeled RGCs in the RE for all four groups were comparable (*Kruskal–Wallis test; p=0.1391). Similarly, the mean total numbers of OHSt-labeled RGCs in the lasered retinas (LE) for these groups were also comparable (**Kruskal–Wallis test; p=0.2991). This suggests that the loss of retrograde axonal transport does not change beyond 8 days.