Figure 5 of O’Brien, Mol Vis 2009; 15:2535-2543.


Figure 5. Relative mRNA content of cells treated with small interfering RNAs to NOX1, NOX4, or NOX5. Human corneal stromal fibroblasts were treated with 40 nM small intefering (si)RNAs to nontarget controls (lanes 1,3, and 5) or NOX1-specific (lane 2), NOX4-specific (lane 4), or NOX5-specific (lane 6) siRNAs. RNA was extracted and amplified in triplicate amplification by real-time reverse transcriptase PCR, using the SYBR Green. Each bar represents the mean and standard error of the mean of triplicate amplifications of RNAs extracted from triplicate cultures. A significant reduction (p<0.05) in the steady state level of RNA was observed for each specific mRNA relative to cultures treated with non-target control RNAs.