Figure 3. Reverse transcriptase PCR products of NADPH oxidase related genes. HCS fibroblasts were grown to confluence and the RNA extracted from cultures and amplified by RT PCR. Sequence specific primers produced the appropriate PCR products for the NADPH oxidase gene products and Rac including A: NOX1, B: NOX4, C: NOX5, D: p22phox, E: p47phox, F: p40phox, G: p67phox, and H: Rac. Row 1: molecular weight standards. Row 2: reverse transcriptase. (RT) PCR products from human corneal stromal fibroblasts; Row 3, no RT controls.