Figure 8. Immunofluorescence detection in differentiated E13.5 and E17.5 RPCs. After 8 days of differentiation, the expression of Brn3b (panel A, B), Map2 (panel C, D), GFAP (panel E, F), glutamine synthetase (panel G, H), and Rhodopsin (panel I, J) in E13.5 and E17.5 RPCs was investigated. K is the statistical ratio of positive cells in both RPCs. Note that the expression ratios of Brn3b and MAP2 in E13.5 RPCs were significantly higher than in E17.5 RPCs. In contrast, E17.5 RPCs expressed GFAP, glutamine synthetase (GS), and rhodopsin in higher percentages. The values were mean±standard deviation from three experiments. The symbols * and ** represent p<0.05 and p<0.01, respectively, versus E13.5 RPCs. Bars were 20 μm. The significance was evaluated by the Student t-test.