Figure 3. Nestin expression and BrdU incorporation in RPCs before and after differentiation. The 4 day proliferation-cultured E13.5 and E17.5 RPCs were of differentiation-cultured. Before and after 8 day of differentiation, Nestin expression and BrdU incorporation were detected. Note that before differentiation, the expression of Nestin in both RPCs was vigorous, but significantly decreased after 8 days of differentiation (A–D, I). Similarly, the incorporation ratio of BrdU in both RPCs decreased dramatically after differentiation (E–H, J). Bars were 20 μm. Data in I and J is mean±standard deviation (n=3). The significance was determined by the Student t-test. The symbol ** represents p<0.01 versus before differentiation.