Figure 2. E13.5 and E17.5 RPCs cultured in proliferation and differentiation conditions. Both RPCs adopted suspension growth and formed neurospheres in proliferation culture (A, C). After starting the differentiation culture, E13.5 and E17.5 RPCs became adherent growth and initiated differentiation by displaying stretched-out processes (B, D). Note that the processes in E13.5 RPCs (B) were long and thick, while in E17.5 RPCs (D) they were mainly short and thin. Bars were 50 μm.