Figure 3. Targeting of the 3’-unstranslated regions of BMP1, ADAM12, and NKIRAS2 by miR-29b. A: Predicted interactions between miR-29b with the 3’-unstranslated region (3’ UTR) of BMP1 (PicTar-Vert), ADAM12 (TargetScan), and NKRAS2 (PicTar-Vert). Seed regions are highlighted in bold. B: Luciferase activity in 293 cells cotransfected with psicheck vectors containing the 3’UTR or complementary sequence (R) from BMP1, ADAM12, or NKIRAS2 and miR-29b or scramble. C: Changes in expression of ADAM12, BMP1, and NKIRAS2 were measured by Q-PCR after transfection with miR-29b mimic or scramble. The figures represent the logarithm of the fold change in gene expression compared to cells transfected with scramble in three different cells lines. Bars represent standard error from three different experiments; one asterisk indicates a p≤0.05, and two asterisks indicate a p≤0.01.