Table 2 of Lee, Mol Vis 2009; 15:2480-2487. Table 2 of Lee, Mol Vis 2009; 15:2480-2487.
Table 2. Characteristics and sequence-specific primers used for RT-PCR and quantitative real-time PCR of 8 differentially expressed genes.
Gene |
GenBank number |
Primer sequence (5'-3') |
Annealing temperature (°C) |
PCR product size (bp) |
|---|---|---|---|---|
| Bone morphogenetic protein 4 (BMP4) | NM_130850 | F: GGGCACCTCATCACACGAC | ||
| R: TGGGCACACAACAGGCTTT | 60 | 550 | ||
| Cofilin 1 (CFL1) | BC011005 | F: TTCTGCGGCTCTCGGTG | ||
| R: GCTTCTCTGCCAGGGTG | 60 | 499 | ||
| JAW1-related protein (MRVI1) | XM_001166452 | F: TGTGGATCTGAAGAGTCC CCCA | ||
| R: ACAGTTCAATGCTAGCACACCTG | 60 | 282 | ||
| Actin, alpha 2 (ACTA2) | BC017554 | F: CCTCCCTTGAGAAGAGTTACG | ||
| R: GAGCAGGAAAGTGTTTTAGAA | 60 | 443 | ||
| Gene rich cluster and C 10 gene (GRCC10) | BC009925 | F; CGTGGCTCTTTATTCGTGA | ||
| R: GGGCTTCGTAGGACTTGAC | 60 | 333 | ||
| Tissue inhibitor of metalloproteinase 3 (TIMP3) | NM_000362 | F: AAGCGATGTCAGAGGGCG | ||
| R: AACTGGATGGGCAGCAGG | 60 | 465 | ||
| Tissue inhibitor of metalloproteinase 1 (TIMP1) | NM_003254 | F: GTTCCAAGCCTTAGGGGATGC | ||
| R: TGACGTAGTCAGGTCCACCAC T | 60 | 407 | ||
| Somatostatin receptor 1 (SSTR1) | NM_001049 | F: CTGGCTTGTACATAGTAGGCAC | ||
| R:CAATGGTAACAAGTTAAGAGCACT | 60 | 426 |