Figure 1. Annealing control primer™–base polymerase chain reaction. Annealing control primer™–base polymerase chain reaction product of eight verified differentially expressed genes shows increased or decreased levels of expression in keratoconus (arrows). Higher levels of expression in keratoconus (column 2) compared with normal cornea (column 1) were found in bone morphogenetic protein 4 (A), cofilin 1 (B), and JAW1-related protein (C), whereas lower levels of expression were found in actin, alpha 2 (D), gene rich cluster, and C 10 gene (E), tissue inhibitor of metalloproteinase 3 (F), tissue inhibitor of metalloproteinase 1 (G), and somatostatin receptor 1 (H).