Figure 5. Chaperone activity with heat denatured b-crystallin. β_L-crystallin (0.3 mg/ml) aggregation in the presence and absence of different concentrations of dogfish α-crystallin. The proteins were incubated in 50 mM sodium phosphate buffer, 0.03% (w/v) NaN₃, pH 7.2 at 60 ^°C. The molar ratios of β_L-crystallin:dogfish α-crystallin are indicated.