Figure 4. Chaperone activity with insulin
reduction. Insulin (0.42 mg/ml) aggregation in the presence and absence
of (A) dogfish and (B) bovine α-crystallins. The proteins
were incubated in 50 mM sodium phosphate buffer, 0.03% (w/v) NaN3,
7.2, at 37 °C. Aggregation of insulin was initiated with
20 mM DTT. The molar ratios of insulin:α-crystallin are indicated.