Figure 2. Thiol reactivity of
a-crystallins. A: Time course for the reaction of dogfish and
bovine α-crystallins with DTNB under pseudo first order conditions and B:
order log transform of the data in A, showing the lines
of best fit for the slowly reacting (k=0.0034 and 0.0048) class of
sulphydryls. C: First order log plot of the differences between
the extrapolated and observed values in B, showing the more
rapidly reacting (k=0.21 and 0.25) class of sulphydryls.