Figure 7. Distribution of 59Fe-labeled ferritin throughout different layers of lens fiber cells from noncataractous and cataractous lenses. Lens fiber cell
homogenates containing 1500 µg of protein were incubated with 59FeCl3 and separated by 8% SDS-PAGE under nonreducing conditions. The mobility of the assembled ferritin complex was compared to
that of 450 kDa canine liver ferritin standards. Incorporation of iron into each layer was expressed as a percent of incorporation
into all layers combined. The autoradiograms shown are of a 1-year-old dog’s noncataractous lens and a 10-year-old dog’s lens
with an early cataract.