Figure 5. SDS-PAGE mobility of the 21-29 kDa ferritin H chains from different layers of an advanced cataractous lens. Fiber cell homogenates from the nucleus and inner cortex (50 µg/sample) were separated by 15% SDS-PAGE under reducing conditions. Anti-canine ferritin H-chain antibodies were used to identify ferritin H chains. Canine heart ferritin was used as a standard (21 kDa).